Lower values remove more low-quality bases.
Bases below this score will be replaced with “N”.
Secondary peaks reaching this percentage of the primary peak’s height will be reported.
How Does It Works?
SangerAnalyst runs quality trimming with Mott's Algorithm, aligns the reads and selects the highest-quality bases to generate a consensus, then removes primer & off-target regions to produce a clean final reports. Powered by Biopython — trusted library for sequence processing and alignment.
